Figure 6.

Rho kinase pathway peptide inhibitors inhibited MLC phosphatase phosphorylation. (a) ROCKs can activate myosin light chain (MLC) by increasing its phosphorylation directly or through deactivating MLC phosphatase (MYPT1) by phosphorylating and inhibiting its myosin-binding subunit (MBS). (b) HUVECs were incubated with 100 µM of TAT-peptide7, 100 µM of TAT peptide, 100 µM of Peptide22, and 10 µM of Y-27632 in the presence or absence of 0.1% DMSO for 12 h. The amount of MYPT1 phosphorylation for each treatment was measured through immunoblotting (n = 3). (A color version of this figure is available in the online journal.)