SIP expression in MMP+ treated C6 cells. C6 cells were treated with various concentrations of MPP+ (0, 100, 250, 500, 750, and 1000 μM) for 24 hours, then SIP mRNA (A) and protein (B) expression level in C6 cells was detected using qRT-PCR and western blotting, respectively. C6 cells were treated with 500 μM MPP+ for indicated times (0, 6, 12, 24, and 48 hours), then SIP mRNA (C) and protein (D) expression level in C6 cells was detected using qRT-PCR and western blotting, respectively. Data were displayed as mean ±SD. *, ** P<0.05, 0.01 versus control. SIP – steroid receptor coactivator-interacting protein; qRT-PCR – quantitative reverse transcription polymerase chain reaction; MPP+ – 1-methyl-4-phenylpyridinium; SD – standard deviation.