Skip to main content
. 2019 Aug 3;25:5776–5784. doi: 10.12659/MSM.912106

Figure 2.

Figure 2

Effect of SIP on MMP+ treated C6 cell viability. (A) C6 cells were transfected with control-plasmid or SIP-plasmid for 24 hours, then mRNA level of SIP was measure by qRT-PCR. (B) C6 cells were transfected with control-plasmid or SIP-plasmid for 24 hours, then protein levels of SIP were measure by western blotting. (C) C6 cells were treated with various concentrations of MPP+ (0, 100, 250, 500, 750, and 1000 μM) for 24 hours, then cell viability was detected using MTT assay. (D) C6 cells were transfected with control-plasmid and SIP-plasmid for 2 hours prior to treatment with 500 μM MPP+ for 24 hours, then cell viability was detected using MTT assay. Data were displayed as mean ±SD. *, ** P<0.05, 0.01 versus control. # P<0.05 versus MMP+ treatment alone group. SIP – steroid receptor coactivator-interacting protein; qRT-PCR – quantitative reverse transcription polymerase chain reaction; MPP+ – 1-methyl-4-phenylpyridinium; SD – standard deviation.