Figure 7.

Effect of SIP on NF-κB pathway in MMP⁺ treated C6 cells. C6 cells were transfected with control-plasmid and SIP-plasmid for 2 hours prior to treatment with 500 μM MPP⁺ for 24 hours, then SIP protein (A) and mRNA (B) level was detected using qRT-PCR and western blotting, and p-p65 protein (A, C) level was measured using western blot assay. Data were displayed as mean ±SD. ** P<0.01 versus control. ^## P<0.01 versus MMP⁺ treatment alone group. SIP – steroid receptor coactivator-interacting protein; NF-κB – nuclear factor-κB; MPP⁺ – 1-methyl-4-phenylpyridinium; qRT-PCR – quantitative reverse transcription polymerase chain reaction; SD – standard deviation.