Table 1. The newly identified conserved cowpea miRNAs characterization. Cowpea miRNAs were characterized in terms of precursor miRNA length (PL), minimum free energy (MFE), mature sequence (MS), number of mismatches (NM) (represented in bold red and enlarged font size), mature sequence length (ML), source EST (SE), mature sequence arm (MSA), GC content percentage (GC%), SL = Strand Location and organ of expression (OE).
| vun miRNAs | Ref. miRNAs | PL | MFE | MS | NM | ML | SE # | MSA | GC% | SL | OE |
| vun-mir398 | mtr-mir398a | 131 | −32.24 | TGTGTTCTCAGGTCGCCCCTG | 2 | 21 | FF542932 | 5′ | 61.90 | + | leaves |
| vun-mir413 | ath-mir413 | 353 | −88.55 | TTAGTTTCTCTTGTTCTGCTT | 2 | 21 | FG940215 | 5′ | 33.33 | + | mixed |
| vun-mir435 | osa-mir435 | 347 | −124.38 | TTATGAGGCTTTGGAGTTGA | 4 | 20 | FG811172 | 3′ | 40.00 | + | mixed |
| vun-mir834 | ath-mir834 | 135 | −52.95 | TGGTAGCAGTGGCGGTGGTGG | 3 | 21 | FG822669 | 3′ | 66.66 | − | mixed |
| vun-mir1512 | gma-mir1512a | 46 | −10.60 | CCTTTAAGAATTTCA-TTA-- | 4 | 18 | FG880488 | 3′ | 22.22 | − | mixed |
| vun-mir1514 | gma-mir1514 | 127 | −31.70 | TTCATTTCTAAAATAGGCATC | 2 | 21 | FF388166 | 5′ | 28.57 | − | root |
| vun-mir1525 | gma-mir1525 | 78 | −14.10 | GGGGTTAAATATGTTTTTAGT | 3 | 21 | FG845219 | 5′ | 28.57 | + | mixed |
| vun-mir1848 | osa-mir1848 | 77 | −32.20 | CGCTCGCCGGCGCGCGCGTCCA | 2 | 22 | FG920123 | 3′ | 86.36 | + | mixed |
| vun-mir2095 | osa-mir2095 | 57 | −17.20 | CTTCCATTTATGACATGTTT | 3 | 20 | FG838629 | 5′ | 30.00 | − | mixed |
| vun-mir2606 | mtr-mir2606a | 69 | −13.00 | TTGAAGTGCTTGGTTCTCACT | 4 | 21 | FG931806 | 5′ | 42.85 | + | mixed |
| vun-mir2609 | mtr-mir2609a | 70 | −13.00 | TTGAAGTGCTTGGTTCTCACT | 4 | 21 | FG931806 | 5′ | 42.85 | + | mixed |
| vun-mir2622 | mtr-mir2622 | 210 | −36.85 | CTTGTGTGCCATTGTGAGCTTA | 3 | 22 | FG900047 | 3′ | 42.85 | − | mixed |
| vun-mir2630 | mtr-mir2630a | 114 | −24.70 | TGGTTTTGGTCTTTGGTTTTA | 3 | 21 | FF391380 | 5′ | 33.33 | + | root |
| vun-mir2636 | mtr-mit2636 | 191 | −29.40 | GGATGTTAGTGTGCTGAATAT | 4 | 21 | FG814033 | 5′ | 38.09 | − | mixed |
| vun-mir2657 | mtr-mir2657 | 156 | −35.38 | TTTTATTGTATTGATTTTGTTG | 4 | 22 | FG926034 | 5′ | 18.18 | − | mixed |
| vun-mir2678 | mtr-mir2678 | 136 | −39.32 | TAAAGTTGTTGCGCGTGTC | 3 | 19 | FF389500 | 3′ | 47.36 | − | root |
| vun-mir2950 | mes-mir2950 | 347 | −83.20 | TTCCATCTCTTGCAGACTGAA | 2 | 21 | FG872933 | 5′ | 42.85 | − | mixed |
| vun-mir3434 | ath-mir3434 | 78 | −17.40 | TGAGAGTATCAGCCATGAGA | 2 | 20 | FF392538 | 3′ | 45.00 | − | root |
| vun-mir4351 | gma-mir4351 | 148 | −63.30 | GTTAGGGTTCAGTTGGAGTTGG | 3 | 22 | FG936300 | 3′ | 50.00 | − | mixed |
| vun-mir4392 | gma-mir4392 | 306 | −80.53 | TCTGTGAGAACGTGATTTCGGA | 3 | 22 | FG857306 | 5′ | 45.45 | + | mixed |
| vun-mir4408 | gma-mir4408 | 66 | −20.70 | CAACAACATTGGATGAGTATAGGA | 4 | 24 | FG894682 | 3′ | 37.5 | + | mixed |
| vun-mir4414a vun-mir4414b |
mtr-mir4414a | 120 | −42.20 | AGCTGCTGACTCGTTGGTTCA ATTCAACGATGCGGGAGCTGC |
0 1 |
21 21 |
FF537171 | 5′ 3′ |
52.38 57.14 |
+ + |
leaves |
| vun-mir4992 | gma-mir4992 | 63 | −21.20 | CATCTAAGATGGTTTTTTTCAG | 4 | 22 | FG926352 | 3′ | 31.81 | − | mixed |
| vun-mir4996 | gma-mir4996 | 163 | −49.83 | TAGAAGTTACCCATGTTCTC | 2 | 20 | FF388735 | 3′ | 40.00 | − | root |
| vun-mir5012 | ath-mir5012 | 172 | −43.44 | TTTTGCTGCTCCGTGTGTTCC | 3 | 21 | FG809429 | 3′ | 52.38 | + | mixed |
| vun-mir5043 | gma-mir5043 | 125 | −48.20 | CTTCTCCTTCTCTGCACCACC | 3 | 21 | FG810406 | 5′ | 57.14 | + | mixed |
| vun-mir5215 | mtr-mir5215 | 181 | −49.63 | AGGAGGATGAGCTAGTTGATT | 3 | 21 | FG939979 | 5′ | 42.85 | + | mixed |
| vun-mir5216 | mtr-mir5216a | 124 | −27.58 | TTGGGAGTGAAAAACAGTGGAA | 2 | 22 | FF399948 | 5′ | 40.90 | + | root |
| vun-mir5219 | mtr-mir5219 | 107 | −25.23 | TCATGGAATCTCAGCTGCAGCAG | 1 | 23 | FG850600 | 3′ | 52.17 | − | mixed |
| vun-mir5227 | mtr-mir5227 | 140 | −18.04 | AGAACAGAAGAAGATTGAAGAA | 3 | 22 | FG915684 | 5′ | 31.81 | − | mixed |
| vun-mir5241 | mtr-mir5241a | 381 | −119.80 | TGGGTGAATGGAAGAGTGAAT | 3 | 21 | FG904590 | 3′ | 42.85 | + | mixed |
| vun-mir5246 | mtr-mir5246 | 68 | −18.70 | CACCAGAGAGCTTTGAAGGTT | 4 | 21 | FG856911 | 3′ | 47.61 | + | mixed |
| vun-mir5255 | mtr-mir5255 | 54 | −10.40 | TGACAGGATAGAGGACATGAC | 4 | 21 | FG910302 | 5′ | 47.61 | − | mixed |
| vun-mir5261 | mtr-mir5261 | 311 | −71.81 | CGATTGTAGATGGCTTTGGCT | 3 | 21 | FG838847 | 5′ | 47.61 | − | mixed |
| vun-mir5280 | mtr-mir5280 | 90 | −20.22 | TAAGTAGAAACGGGCCGAGATCGGGG | 4 | 26 | FG915361 | 5′ | 57.69 | − | mixed |
| vun-mir5290 | mtr-mir5290 | 217 | −30.24 | AAAGTAGAGAGAGAAAGACACATA | 4 | 24 | FG852502 | 5′ | 33.33 | + | mixed |
| vun-mir5298 | mtr-mir5298a | 192 | −36.58 | TGGATTTCAAGATGAAGATGAAGAA | 4 | 25 | FF402284 | 3′ | 32.00 | − | root |
| vun-mir5376 | gma-mir5376 | 341 | −132.02 | TGGAGATTGTGAAGAATTTGAGA | 3 | 23 | FG872123 | 3′ | 34.78 | + | mixed |
| vun-mir5561 | mtr-mir5561 | 346 | −69.34 | ATCTCTCTCTCTCTAAATGTA | 3 | 21 | FF390124 | 5′ | 33.33 | − | root |
| vun-mir5758 | mtr-mir5758 | 91 | −22.60 | TAAGTTGGATCTATGTATTTG | 3 | 21 | FG893334 | 3′ | 28.57 | + | mixed |
| vun-mir5770 | gma-mir5770a | 98 | −30.40 | TTAGGACTATGGTTTGGATGA | 1 | 21 | FG937135 | 3′ | 38.09 | − | mixed |
| vun-mir6252 | osa-mir6252 | 90 | −20.90 | ATGAGTTGTGTTGAGAGAGGGTT | 4 | 23 | FG841373 | 3′ | 43.47 | − | mixed |
| vun-mir7696 | mtr-mir7696a | 173 | −33.67 | ACAAGTACTTA-AATTCAAAA | 4 | 20 | FG864277 | 3′ | 20.00 | − | mixed |
| vun-mir8182 | ath-mir8182 | 170 | −31.80 | TTGTGTTGCGTTTGTGATGACT | 3 | 22 | FG942892 | 5′ | 40.90 | − | mixed |
| vun-mir9748 | gma-mir9748 | 98 | −32.45 | GAAGGAAGTGTTGAGGGAGGAG | 3 | 22 | FG921211 | 5′ | 54.54 | + | mixed |