Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2019 Aug 5;9:716. doi: 10.3389/fonc.2019.00716

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright © 2019 Agorku, Langhammer, Heider, Wild, Bosio and Hardt.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

PMC Copyright notice

Ex vivo analysis of normal fibroblasts from tumor-bearing mice. (A–C) Comparison of NFs derived from tumor-bearing mice with “healthy” NFs and CAFs. Fibroblasts were isolated and analyzed ex vivo for their expression of CD39, CD87, Sca-1, CD95, Ly-6C, CD44, and CD49b. NFs from tumor-bearing mice were isolated from tumor-free skin and mammary fat pad of the flank where no tumor cells had been injected (A), thus they have not been in direct contact with tumor cells. As healthy NF control population, fibroblasts were isolated from healthy BALB/c mice. The phenotype of NFs from tumor bearing mice was analyzed in CT26.WT (B) and 4T1 (C) mouse tumor model. The percentage of cells expressing the respective marker is depicted. Data are reported as median and SEMs for three replicates from at least two independent experiments and statistical analysis was performed using the two-way ANOVA (^**P < 0.005; ^***P < 0.0005; ^****P < 0.0001).