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Representative MS/MS spectrum of a doubly-charged ion (m/z 794.87) corresponding to the peptide sequence of 182-TTCLPMFGYK-191 with a biotin-HPDP modification at Cys184 in the β3 subunit. The observed y- and b-ion series confirmed the peptide sequence, and a biotin-HPDP modified Cys (+428.2 amu) found between b2 and b3 as well as between y7 and y8 ions in the spectrum confirmed the modification of Cys184.
