FIG 1.
Pool selection and top-down tandem mass spectrometry (MS/MS) workflow to keep intact proteins. The intact-protein analysis system (IPAS) compared graft-versus-tumor (GVT)–positive and graft-versus-host disease (GVHD)–negative post–donor lymphocyte injection (DLI; heavy isotope) with GVT-positive and GVHD-positive post-DLI (light isotope) samples in a training set (IPAS-01), and GVT-positive and GVHD-negative post-DLI (heavy isotope) with GVT-positive and GVHD-negative pre-DLI (light isotope) samples in a validation set (IPAS-02).