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. Author manuscript; available in PMC: 2020 May 1.
Published in final edited form as: Biotechnol Bioeng. 2019 Jan 29;116(5):1220–1230. doi: 10.1002/bit.26921

Figure 3.

Figure 3.

PBAE polymers are not prone to freeze-thaw induced reductions in efficacy. (A) Schematic of experimental outline for testing effect of freeze-thaw cycles on polymer efficacy. (B) HEK293T and (C) MDA-MB-231 cell transfection efficacy were not statistically different even with PBAE 4–4-6 having undergone eight freeze-thaw cycles (one-way ANOVA with multiple comparisons to the fresh polymer). Similarly, PBAE 4–4-6 aliquots pre-mixed to 50 mg/mL prior to storage with varying degrees of hydrated DMSO showed no loss in efficacy when stored at −20°C for two weeks and then used within an hour after thawing in (D) HEK293T or (E) MDA-MB-231 cells.