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. Author manuscript; available in PMC: 2020 May 1.
Published in final edited form as: Biotechnol Bioeng. 2019 Jan 29;116(5):1220–1230. doi: 10.1002/bit.26921

Figure 5.

Figure 5.

Acetate anion buffers with divalent magnesium or calcium cations improve transfection efficacy over monovalent sodium acetate buffer. PBAEs 4–4-6 and 4–5-39 had statistically higher transfection efficacy with MgAc2 and CaAc2 buffers than NaAc in (A) HEK293T cells and (B) MDA-MB-231 cells, shown as normalized to the level of transfection efficacy in pH 5, 25 mM NaAc buffer. (C) Microscopy showed increase in geometric mean expression of HEK293T cells using divalent cation acetate buffers. Results show multiple experiments with four replicates each, normalized to NaAc buffer transfection efficacy for that experiment. Scale bars 200 μm.