Fig 3. Hypertension and cardiac fibrosis.

3(a) Cardiac transverse sections, taken 6mm from the apex (scale bar is 3mm) from normotensive animals (N) after three months, hypertensive animals (H) and hypertensive animals with spironolactone (H+SP) after both one and three months. Sections were stained with picrosirius red and light green counter stain. Inlay sections (50x magnification, scale bar 100μm) are taken from the lateral wall of the left ventricle. (3b, 3c) Percentage of fibrosis of the left ventricle (transverse sections taken 6mm from the apex) from normotensive (N, grey, n = 8), hypertensive (H, black, n = 8) and hypertensive animals dosed daily with spironolactone (H+SP, striped, n = 4) following one month (b) and three months (c). Values for normotensive animals were taken at three months only (dashed line), and were compared with hypertensive and spironolactone treated animals at both one month and at three months. Values are shown as mean ± standard deviation.