Fig 4.

Effects of Ethanol with or without Raspberry Ketone/Omeprazole on the Gastric Nrf2 and HMGB1 protein level (A) Representative immunoblot of protein level from liver tissue. Control: normal control rats; E: untreated ethanol-ulcerated group; RK+E: Ethanol ulcerated group pretreated with Raspberry Ketone (50 mg/kg, p.o.); Omep+E: Ethanol ulcerated group pretreated with Omeprazole (20 mg/kg, p.o.); RK: Rats treated with Raspberry Ketone alone (50 mg/kg, p.o.). Sample proteins were resolved by SDS-PAGE and immunoblotted with HMGB1 antibody "upper panel", Nrf2 antibody "middle panel", and after stripping with β-actin antibody as the loading control "lower panel". The densities of immunoblots were quantified using analysis software. The sizes of the molecular mass markers expressed in kDa are indicated at the right of the panel. (B) Quantitative results of the immunoblots of Nrf2 (n = 3). (C) Quantitative results of the immunoblots of HMGB1. Protein levels are expressed as the ratio to β-actin. The relative quantities were normalized to the control and expressed as a fold of induction. Data are presented as the mean ± SEM. a, b: Significantly different from the control group, and ethanol group, respectively at P <0.05 using one way ANOVA followed by post hoc Tukey Kramer test. (D) Represents the correlation line and equation between Nrf2 and HMGB1 protein level.