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. 2019 Aug 8;14:6357–6369. doi: 10.2147/IJN.S214727

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© 2019 Zeng et al.

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Preparation of IL-12 loaded nanoparticles (IL-12-PNP). IL-12-PNP was prepared by the double emulsion method. In brief, the initial W1/O (water/oil) emulsion was prepared by dispersing the inner phase (IL-12 solution) via sonication into the disperse phase with ethyl acetate solution dissolved in PLGA polymer. The W1/O emulsion was injected into PVA solution to form the W1/O/W2 emulsion which was then mechanically stirred to evaporate the organic solvent, washed, and freeze-dried to yield the final nanoparticles.