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. Author manuscript; available in PMC: 2019 Aug 12.
Published in final edited form as: Cell Rep. 2019 Jul 9;28(2):472–485.e5. doi: 10.1016/j.celrep.2019.06.029

Figure 3. MTA2/NuRD Interacts with AIOLOS and Represses Igll1 and VpreB Genes in Pre-B Cells.

Figure 3.

(A) Immunoprecipitation (IP)-Western blot results using 697 human pre-B leukemia cell nuclear extract (NE). Antibodies used for IPs and immunoblots are marked (AIO: AIOLOS, IKA: IKAROS).

(B) RT-qPCR results showing expression levels of Igll1 and VpreB1 genes in pre-B cells isolated from Vav-Cre Mta2fl/fl, Mx1-Cre Mta2fl/fl and their litter-mate control mice (n = 4 for Vav-Cre Mta2fl/fl and control, n = 3 for Mx1-Cre Mta2fl/fl and control; t test; *p < 0.05, **p < 0.01, ***p < 0.001).

(C) ChIP-qPCR data using human 697 cells showing enrichment of AIOLOS, MI-2β, MTA2 (left panel), and IKAROS (right panel) at the IGLL1 and VPREB1 promoter regions. The CNAP1 gene locus was used as a negative region control. Data were collected from 3 or more independent ChIP assays.

(D) ChIP-qPCR data showing enrichment of H3K27Ac and H3K4Me3 marks (normalized to H3) at promoter regions of Igll1 and VpreB1 genes in primary small pre-B cells sorted from Vav-Cre Mta2fl/fl (VavKO) and control mice (t test; *p < 0.05, **p < 0.01).