KEY RESOURCES TABLE
| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Antibodies | ||
| PE/Cy7 anti-mouse/human CD45R/B220 | BioLegend | Cat# 103222; RRID: AB_313005 |
| PE anti-mouse IgM | BioLegend | Cat# 406508; RRID: AB_315058 |
| APC anti-mouse IgM | BioLegend | Cat# 406509; RRID: AB_315059 |
| FITC anti-mouse CD43 | BioLegend | Cat# 143204; RRID: AB_10960745 |
| FITC anti-mouse CD21/CD35 (CR2/CR1) | BioLegend | Cat# 123407; RRID: AB_940403 |
| PE anti-mouse CD23 | BioLegend | Cat# 101607; RRID: AB_312832 |
| Biotin anti-mouse Ly-51 (6C3/BP-1) | BioLegend | Cat# 108303; RRID: AB_313360 |
| PE Streptavidin | BioLegend | Cat# 405203; RRID: N/A |
| APC anti-mouse CD24 (HAS) | BioLegend | Cat# 138505; RRID: AB_2565650 |
| Brilliant Violet 605 anti-mouse IgM | BioLegend | Cat# 406523; RRID: AB_2563358 |
| PerCP/Cyanine5.5 anti-mouse CD95(Fas) | BioLegend | Cat# 152610; RRID: AB_2632905 |
| APC anti-mouse CD38 | BioLegend | Cat# 102712; RRID: AB_312933 |
| APC anti-mouse CD4 | BioLegend | Cat# 116014; RRID: AB_2563025 |
| PE anti-mouse IgG1 | BioLegend | Cat# 406607; RRID: AB_10551439 |
| Biotin anti-mouse IgG3 | BioLegend | Cat# 406803; RRID: AB_315070 |
| Biotin anti-mouse IgA | BioLegend | Cat# 407003; RRID: AB_315078 |
| Anti-mouse CD40 | BioLegend | Cat# 102802; RRID: AB_312935 |
| Anti-Mta2 | Abcam | Cat# ab8106; RRID: AB_306276 |
| Anti-CHD4 (Mi-2β) | Abcam | Cat# ab72418; RRID: AB_1268107 |
| Anti-IKZF3 (Aiolos) | Abcam | Cat# ab139408; RRID: N/A |
| Anti-IKZF1 (Ikaros) | Abcam | Cat# ab 26083; RRID: AB_881148 |
| Anti-Bob1 (Oca-B) (C-20) | Santa Cruz Biotechnology | Cat# sc-955; RRID: AB_2166917 |
| Anti-Oct-2 (C-20) | Santa Cruz Biotechnology | Cat# sc-233; RRID: AB_2167205 |
| Anti-HDAC2 | Abcam | Cat# ab7029; RRID: AB_305706 |
| Anti-Histone H3 (tri methyl K4) | Abcam | Cat# ab8580; RRID: AB_306649 |
| Anti-Histone H3 (acetyl K27) | Abcam | Cat# ab4729; RRID: AB_2118291 |
| Anti-Histone H3 | Abcam | Cat# ab1791; RRID: AB_302613 |
| Normal rabbit IgG | Santa Cruz Biotechnology | Cat# sc-2027; RRID: AB_737197 |
| Chemicals, Peptides, and Recombinant Proteins | ||
| DSG (disuccinimidyl glutarate) | Thermo Fisher Scientific | Cat# 20593 |
| Recombinant Murine IL-4 | PeproTech | Cat# 214–14 |
| Recombinant TGF-β (carrier free) | BioLegend | Cat# 763102 |
| Critical Commercial Assays | ||
| Arcturus™ PicoPure RNA isolation Kit | Applied Biosystems | Cat# KIT0204 |
| qScript cDNA SuperMix | Quanta Biosciences | Cat# 95048 |
| DNeasy Blood and Tissue Kit | QIAGEN | Cat# 69506 |
| QuantiTect SYBR Green PCR mix | QIAGEN | Cat# 204145 |
| JumpStart TaqReady Mix | Sigma-Aldrich | Cat# P2893 |
| End-It™ End-Repair Kit | Epicenter | Cat# ER81050 |
| DNA Clean & Concentrator Kit | Zymo Research | Cat# D4014 |
| NEXTflexChIP-Seq Barcodes | Perkin Elmer | Cat# NOVA-514120 |
| Dynabeads™ Protein G | Invitrogen | Cat# 10004D |
| TruSeq Stranded mRNA Library Prep Kit | Illumina | Cat# 20020594 |
| Mouse CD43 MicroBeads | Miltenyi Biotec | Cat# 130-049-801 |
| Deposited Data | ||
| Mta2 Control_(781t4)_pro-B | This Paper | GSM3398870 |
| Mta2 Control_(789t7)_pro-B | This Paper | GSM 3398871 |
| Vav-Cre Mta2 f/f _(781t3)_pro-B | This Paper | GSM 3398872 |
| Vav-Cre Mta2 f/f _(789t6)_pro-B | This Paper | GSM3398873 |
| Mta2 Control_(918t3)_pre-B | This Paper | GSM3398874 |
| Mta2 Control_(919t7)_pre-B | This Paper | GSM3398875 |
| Mta2 Control_(931t10)_pre-B | This Paper | GSM3398876 |
| Vav-Cre Mta2 f/f _(918t4)_pre-B | This Paper | GSM3398877 |
| Vav-Cre Mta2 f/f _(918t6)_pre-B | This Paper | GSM3398878 |
| Vav-Cre Mta2 f/f _(931t14)_pre-B | This Paper | GSM3398879 |
| INPUT_697 | This Paper | GSM2882822 |
| AIOLOS_697 | This Paper | GSM2882823 |
| IKAROS_697 | This Paper | GSM2882824 |
| MTA2_697 | This Paper | GSM2882825 |
| Mi2_697 | This Paper | GSM2882826 |
| OCAB_697 | This Paper | GSM2882827 |
| OCT2_697 | This Paper | GSM2882828 |
| HDAC2_697 | This Paper | GSM3398902 |
| Experimental Models: Cell Lines | ||
| 697 human pre-B acute lymphoblastic leukemia cell line | DSMZ | Cat# ACC42 |
| Namalwa Burkitt’s lymphoma cell line | ATCC | Cat# CRL-1432™ |
| Experimental Models: Organisms/Strains | ||
| Mta2 conventional null mice | Lu et al.,2008 | N/A |
| Cd19-Cre transgenic mice | The Jackson Lab | Stock # 004126 |
| Mb1-Cre transgenic mice | The Jackson Lab | Stock # 020505 |
| Mx-Cre transgenic mice | The Jackson Lab | Stock # 003556 |
| Vav1-Cre transgenic mice | The Jackson Lab | Stock # 008610 |
| Oca-B null mice | Kim et al., 1996 | N/A |
| Oligonucleotides | ||
| sh_hOCA-B#1 | Sigma-Aldrich | Cat# TRCN0000431764 |
| 5′-AAATG AAAG CCCATG G ACCAC-3′ | ||
| Sh_hOCA-B#2 | Sigma-Aldrich | Cat# TRCN0000423580 |
| 5′-AAATCCCTATAATTACCTCCC-3′ | ||
| Scramble shRNA | Sigma-Aldrich | Cat# SHC002 |
| 5′-CAACAAGATGAAGAGCACCAA-3′ | ||
| Oligonucleotides for qPCRs | This paper | Table S6 |
| Recombinant DNA | ||
| pLKO.1 scramble shRNA | This paper | N/A |
| pLKO.1 sh_hOCA-B#1 | This paper | N/A |
| pLKO.1 sh_hOCA-B#2 | This paper | N/A |
| Software and Algorithms | ||
| Bowtie2 | Langmead and Salzberg, 2012 | http://bowtie-bio.sourceforge.net/bowtie2/index.shtml |
| TopHat | Kim et al., 2013 | https://github.com/DaehwanKimLab/tophat2 |
| Cufflinks | Trapnell et al., 2010 | https://github.com/cole-trapnell-lab/cufflinks |
| edgeR | Robinson et al., 2010 | http://bioconductor.org |
| Enrichr | Kuleshov et al., 2016 | http://amp.pharm.mssm.edu/Enrichr |
| Samtools | Li et al.,2009 | http://samtools.sourceforge.net/ |
| Homer | Heinz et al., 2010 | http://homer.ucsd.edu/homer/ |
| ngsplot | Shen et al., 2014 | https://github.com/shenlab-sinai/ngsplot |
| Integrative Genomics Viewer (IGV) | Robinson et al., 2011 | https://software.broadinstitute.org/software/igv/ |
| GSEA | Subramanian et al., 2005 | http://software.broadinstitute.org/gsea/index.jsp |
| Other | ||
| Sheep Red Blood Cells | Cocalico Biologicals, Inc. | N/A |
| Lipopolysaccharides (LPS) | Sigma-Aldrich | Cat# L2637 |
| Poly (I:C) | Sigma-Aldrich | Cat# P9582 |