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. 2019 Aug 3;21(9):882–892. doi: 10.1016/j.neo.2019.07.005

Figure 3.

Figure 3

SOX4 is a direct target of miR-130a. (A) Venn diagram showing the overlap between predicted target genes of miR-130a from miRDB, Target Scan and RNA sequencing profiling generated from SNU70 and SNU1411 cells. The nine overlapping genes were presented. (B and C) SOX4 expression levels in SNU70, SNU70RR and SW1411 cells were measured with real time PCR (B) and western blotting (C). The data were normalized with cyclophilin as the internal control for real time PCR. (D) Schematic illustration for a potential miR-130a binding site of the SOX4 3′UTR. The mutant sequence (MT) of the SOX4 3′UTR was presented by comparing it with the wild-type sequence (WT). (E) Luciferase assay was performed after transfection with the wild-type or mutant construct in SNU70RR cells. After incubation for 48 h, relative luciferase activity was determined with β-galactosidase as a normalization control. Data are presented as the mean ± S.D. of three independent experiments. (F) The level of SOX4 was measured in SNU70RR-miR-130a cells with western blotting. β-actin was used as the loading control. (⁎⁎P < .01).