Figure 6.
miR-130a overcomes the radioresistance of in vivo rectal cancer in a xenograft model. (A) The growth curves of SNU70RR-control, miR-130a and miR-130a/SOX4 xenograft tumors without or with irradiation (IR) treatment. Mice were locally delivered a single 15 Gy dose of irradiation. After the tumor size reached 1500 mm3, the mice were scarified. The tumor size was measured with caliper every 3 days (n = 6 mice per group). (B) Representative images of tumors after sacrifice. The tumors from the miR-130a expressing cells were markedly smaller after irradiation compared with other cells. (C) Left, immunohistochemical staining for Ki-67 for SNU70RR-scramble, SNU70RR-miR-130a and SNU70RR-miR-130a/SOX4 xenograft tumors. Right, a graph showing the percentage of Ki-67 positive nuclei. (D) Left, representative image of H&E staining for SNU70RR-scramble, SNU70RR-miR-130a and SNU70RR-miR-130a/SOX4 xenograft tumors with the area of cell death. Right, a graph showing the percentage of cell death areas in the tumors. The images were captured and analyzed from at least five different fields. The scale bar represents 50 μm. (⁎⁎P < .01).