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. 2019 Aug 6;10(47):4868–4879. doi: 10.18632/oncotarget.27115

Figure 3. AUF1 enhances the capacity of osteosarcoma cells in promoting endothelial differentiation and angiogenesis in a VEGF-A-dependent manner.

Figure 3

(A and E) SFCM were collected from the indicated cells and were applied independently on HUVEC cells plated on matrigel-coated 96-well plate, using SFM as negative control. The differentiation into capillary- like structures was assessed after 5 hrs of incubation. Representative photographs of HUVEC cavities are shown. Scale bars represent 30 μm. (B and F) Histogram shows average number of microvessels observed in five different fields. Error bars represent means ± SD. *** P ≤ 8.5×10-7. (C) In-vivo matrigel plug assay. SFCM were collected from 48 hrs cultures of the indicated cells, centrifuged and mixed with phenol-red-free matrigel, and were injected subcutaneously into nude mice. Mice were then sacrificed after 8 days and the matrigel plugs were extracted for staining with the CD31 antibody and for haemoglobin content (D).