Figure 2. P2X7R mRNA expression after exposure to H₂O₂ and 5% FBS DMEM.

P2X7R mRNA expression was determined by qPCR after 24 h of treatment with (A) 500 nM H₂O₂ (B) 5% FBS DMEM. Data are averages ± SD from at least three similar experiments. Data were analyzed by Student’s t-test ^*Significantly different from control (no H₂O₂ or cells cultured in 15% FBS), p <0.05. (C) Representative trace showing intracellular calcium increase following stimulation with 500 μM BzATP.