Figure 3. Characterization of the P2X7R receptor in mouse GL261 glioma cells.
(A) P2X7R mRNA expression was measured by qPCR. Human U138-wt cells were used for comparison. Data are averages ± SD from at least three similar experiments analyzed by Student’s t-test. p <0.05. *Significantly different from U138-wt. (B) Representative trace showing intracellular calcium increase following stimulation with 100 μM BzATP in presence or absence of 25 μM A740003. (C) Representative trace showing ethidium bromide uptake triggered by stimulation with 200 μM BzATP. Traces are representative of at least three similar obtained in different occasions.