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. 2019 Aug 6;10(47):4840–4856. doi: 10.18632/oncotarget.27106

Figure 5. P2X7R expression does not affect in vitro glioma cell proliferation in low glucose DMEM.

Figure 5

Cell proliferation was analyzed in U138-wt cells, U138-P2X7 polyclone and U138-P2X7 clone incubated for 24 h, 48 h and 72 h in DMEM containing 1 g/L glucose (low glucose) under the following conditions: (A) 15% FBS DMEM; (B) FBS-free DMEM; (C) absence or presence of 10 μM A740003 in FBS-free DMEM; (D) in the presence of 100 μM BzATP in FBS-free DMEM; (E) in the presence of 10 U/mL apyrase in FBS-free DMEM. In panel F the effect of A740003 (30 μM) in GL261 cultured in 15% FBS DMEM is shown. Data were analyzed by ANOVA followed by Tukey test (p < 0.05). All experiments were carried out at least three times.