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. 2019 Aug 6;10(47):4808–4821. doi: 10.18632/oncotarget.27093

Figure 1. EL-4 cells were cultured with activated lymphocytes transduced with 1D1α and 1D1α-gfp constructions.

Figure 1

Analysis of the number of EL-4 cells (A) and splenic T-cell phenotype (B) were performed 24h after co-culturing. (A) The percentages of Kb positive cells were determined by flow cytometry in three groups of mixed cultures in vitro – control (R101 + EL-4) and two experimental (1D1α + EL-4 and 1D1α-gfp + EL-4). (B) The bar graph represents the ratio of CD4Vα11+, CD4Vα11–, CD8Vα11+, and CD8Vα11– in the culture of T cells expressing 1D1α-gfp without EL-4 relative to the culture of T cells expressing 1D1α-gfp along with EL-4. We define 1D1α-gfp positive cells as Vα11+ because GFP matches the cells expressing α-chain 1D1– a member of the Vα11 protein family. The data represent the mean ± sd (n = 4–6).