Table 1.
Ligand-binding analysis by differential scanning fluorimetry
The means and the standard deviations of the measurements of the unfolding temperatures (Tm) for each protein complex are given.
| Protein complex | Unfolding temperature (Tm) in °C | References |
|---|---|---|
| hAPRT | 49.6 ± 1.2 | 6 |
| hAPRT + Hx | 47.6 ± 0.5 | This work |
| hAPRT + ADE | 50.3 ± 0.6 | 6 |
| hAPRT + PRPP | 79.6 ± 0.6 | 6 |
| hAPRT + PRPP + Hx | 79.7 ± 0.5 | This work |
| hAPRT + PRPP + ADE | 79.9 ± 0.5 | 6 |
| hAPRT + PPi | 52.1 ± 1.0 | This work |
| hAPRT + AMP | 59.0 ± 0.6 | 6 |
| hAPRT + IMP | 51.2 ± 1.1 | This work |
| hAPRT + GMP | 53.0 ± 0.7 | This work |
| hAPRT + PPi + AMP | 64.3 ± 0.7 | 6 |
| hAPRT + PPi + IMP | 53.5 ± 0.7 | This work |
| hAPRT + PPi + GMP | 53.6 ± 1.0 | This work |