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Purified 20β-HSDH from B. adolescentis, strain L2-32. A, SDS-polyacrylamide gel of crude and purified 20β-HSDH showing a subunit size of 32 ± 0.12 kDa. Lane M, molecular mass markers. B, native molecular size analysis of purified 20β-HSDH via gel-filtration chromatography.
