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Steady-state kinetic parameters of 20β-HSDH and active-site mutants
| Protein | Vmax | Km | kcat | Relative kcat | kcat/Km | Relative kcat/Km |
|---|---|---|---|---|---|---|
| μmol·min−1·mg−1 | μm | min−1 | % | μm−1·min−1 | % | |
| Wildtype | 21.08 ± 1.13a | 24.07 ± 3.80 | 668.30 ± 50.54 | 100 | 27.77 ± 3.65 | 100 |
| S181A | b | – | – | – | – | – |
| S183A | 4.70 ± 0.10 | 9.49 ± 2.60 | 148.70 ± 11.24 | 22 | 15.68 ± 2.06 | 56 |
a Values represent the means ± S.D. based on three or more replications.
b – means no activity was detected.
