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. 2019 Jun 27;294(32):11952–11959. doi: 10.1074/jbc.AC119.008361

Figure 2.

Figure 2.

CLCF1 activates the phosphorylation of STAT1 and STAT3 in MSCs. A and B, MSCs were stimulated with CLCF1 (100 ng/ml), CLCF1-mut1 (100 ng/ml), or CLCF1 (100 ng/ml) with or without ruxolitinib (lane CLCF1 + Ruxo; 10 μm) for 15 min. Lysates were subjected to Western blot analysis using anti- phospho-STAT3 (p-STAT3), anti-STAT3, anti-phospho-STAT1 (p-STAT1), or anti-STAT1 mAb, respectively. Signals were revealed using horseradish peroxidase–labeled secondary antibody and chemiluminescence. C, MSCs were stimulated with 0, 0.1, 1, 10, 50, or 100 ng/ml CLCF1 or with 1 ng/ml LIF for 15 min. Lysates were analyzed for phospho-STAT3 and total STAT3 levels as described above.