Table 1.

HSC70 recognizes a YYIYVI sequence in CTA1

Peptides containing overlapping sequences of amino acids from CTA1 were appended to SPR sensor slides. HSC70 was then perfused over each slide; binding to the peptide-coated sensor is indicated by a + sign. A mutant (m) CTA1 sequence is denoted in bold.

CTA1 residues	Peptide sequence	HSC70 interaction
1–16	NDDKLYRADSRPPDEI	−
11–26	RPPDEIKQSGGLMPRG	−
21–36	GLMPRGQSEYFDRGTQ	−
31–46	FDRGTQMNINLYDHAR	−
41–56	LYDHARGTQTGFVRHD	−
51–66	GFVRHDDGYVSTSISL	−
63–78	SISLRSAHLVGQTILS	−
73–88	GQTILSGHSTYYIYVI	+
83–98	YYIYVIATAPNMFNVN	+
93–108	NMFNVNDVLGAYSPHP	−
103–118	AYSPHPDEQEVSALGG	−
113–128	VSALGGIPYSQIYGWY	−
123–138	QIYGWYRVHFGVLDEQ	−
133–148	GVLDEQLHRNRGYRDR	−
143–158	RGYRDRYYSNLDIAPA	−
153–168	LDIAPAADGYGLAGFP	−
163–177	GLAGFPPEHRAWREE	−
173–188	AWREEPWIHHAPPGCG	−
179–192	WIHHAPPGCGNAPR	−
73–82	GQTILSGHST	−
73–88m	GQTILSGHSTYYLYVL	−