Figure 5.

S-Acylation of BK channel α-subunit by Zdhhc23 in murine VSMC. A, representative blots and bar chart (n = 3) from an acyl-RAC experiment of native BK channel α-subunit S-acylation in mouse VSMCs from WT and Zdhhc23^−/− mice. B, RT-qPCR of BK channel α- or β1-subunit and Zdhhc23 mRNA expression in Zdhhc23^−/− VSMCs expressed relative to WT levels (n = 6). C, representative confocal images of immunocytochemical labeling for Zdhhc23 protein (green) and nuclei stained with Hoechst (blue) in WT and Zdhhc23^−/− VSMCs. Scale bars are 5 μm. All data are means ± S.E.