Figure 4.

HPAEC-PAD chromatograms of NcAA13 and MtAA13. A and B, activity assays of NcAA13 (A) and MtAA13 (B) with amylose (blue lines), amylopectin (red lines), and cornstarch (green lines) substrates. A chromatogram of malto-aldonic acid standards (yellow lines) has been included for reference. For clarity, the chromatograms for the amylose assays were scaled down by 10-fold. A1–A30 indicate the malto-aldonic acid products with degrees of polymerization of 1–30. Chromatograms for NcAA13ΔCBM, NcAA13cat, and MtAA13+CBM can be found in the supporting information.