Table 2.
Top five peptides identified from each of 10 abundant lens proteins
Lens peptides were isolated from the gel filtration HMW fraction and identified as described under “Experimental Procedures.” The five peptides with the highest ion scores from each of the proteins listed in Table 1 are displayed with their corresponding theoretical mass and relative abundances in a 44-year-old and 76-year-old whole-lens sample on a scale of 0–200.
| Parent protein | Sequence | Theoretical MH+(Da) | Relative abundances |
|
|---|---|---|---|---|
| 44-Year-old | 76-Year-old | |||
| αA crystallin | 52LFRTVLDSGISEVR65 | 1591.88023 | 2.2 | 197.8 |
| 56VLDSGISEVR65 | 1074.57896 | 12.9 | 187.1 | |
| 134SADGMLTFCGPKIQ147 | 1524.71851 | 6 | 194 | |
| 54RTVLDSGISEVR65 | 1331.72775 | 68.3 | 131.7 | |
| 51SLFRTVLDSGISEVR65 | 1678.91226 | 200 | ||
| αB crystallin | 93VLGDVIEVH101 | 980.54112 | 57.2 | 142.8 |
| 29GEHLLESDLFPT40 | 1357.66342 | 9.8 | 190.2 | |
| 123RIPADVDPLTIT134 | 1310.73144 | 2.2 | 197.8 | |
| 123RIPADVDPLTITS135 | 1397.76347 | 2.7 | 197.3 | |
| 95GDVIEVH101 | 768.38864 | 200 | ||
| βA3 crystallin | 29GPWKITIYD37 | 1092.57242 | 200 | |
| 152GWFNNEVGSMKIQ164 | 1509.71547 | 200 | ||
| 127TIFEKENFIGRQ138 | 1481.7747 | 16 | 184 | |
| 124SKMTIF129 | 726.38547 | 1.4 | 198.6 | |
| 139WEISDDYPSLQAM151 | 1554.67809 | 0.4 | 199.6 | |
| βA4 crystallin | 108TIFEQENFLGKK119 | 1453.76856 | 200 | |
| 109IFEQENFLGKK119 | 1352.72088 | 1.4 | 198.6 | |
| 120GELSDDYPSLQAM132 | 1425.62024 | 1.8 | 198.2 | |
| 105SRLTIFEQENFLGKK119 | 1809.98576 | 200 | ||
| 104DSRLTIFEQENFLGKK119 | 1925.0127 | 200 | ||
| βB1 crystallin | 238HLEGSFPVLA247 | 1069.56767 | 200 | |
| 238HLEGSFPVLATEPPK252 | 1621.85843 | 21 | 179 | |
| 240EGSFPVLATEPPK252 | 1371.71546 | 2.3 | 197.7 | |
| 242SFPVLATEPPK252 | 1185.6514 | 54.3 | 145.7 | |
| 38TLAPTTVPITSAK50 | 1299.75184 | 22.2 | 177.8 | |
| βB2 crystallin | 121KMEIIDDDVPSFHAH135 | 1753.8214 | 200 | |
| 121KMEIIDDDVPSFHA134 | 1616.76248 | 200 | ||
| 121KMEIIDDDVPSFHAHG136 | 1810.84286 | 200 | ||
| 122MEIIDDDVPSFHAH135 | 1625.72643 | 200 | ||
| 143SVRVQSGTWVGYQYPGYRGL162 | 2273.14618 | 200 | ||
| Filensin | 79GELAGPEDALARQVE93 | 1554.77583 | 66.3 | 133.7 |
| 324FIETPIPLFTQSH336 | 1529.79986 | 200 | ||
| 239RVELQAQTTTLEQAIK254 | 1829.0127 | 5.8 | 194.2 | |
| 324 FIETPIPLFTQ334 | 1305.70892 | 1.1 | 198.9 | |
| 191QQIIHTTPPASIVTS205 | 1592.86425 | 2.6 | 197.4 | |
| γS crystallin | 60YILPQGEYPEYQRWM74 | 1972.9262 | 200 | |
| 98IFEKGDFSGQ107 | 1127.53677 | 200 | ||
| 65GEYPEYQRWM74 | 1358.5834 | 13.2 | 186.8 | |
| 50YERPNFAGYM59 | 1247.55137 | 200 | ||
| 98IFEKGDFSGQMYETTED114 | 1996.84806 | 10 | 190 | |
| Phakinin | 162QQVGEAVLENARL174 | 1426.76487 | 200 | |
| 157WASSCQQVGEAVLENARL174 | 2017.976 | 200 | ||
| 211KVIDEANLTKM221 | 1261.68205 | 6.3 | 193.7 | |
| 266TGLDDILETIRIQ278 | 1486.81115 | 200 | ||
| 162QQVGEAVLENARLM175 | 1557.80535 | 4 | 196 | |
| γC crystallin | 166SLRRVVDLY174 | 1120.64732 | 200 | |