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N-terminal region of CDK4 is required for A3B binding. A, amino acid alignment of CDK4 and CDK2 with chimeric junctions indicated in red and structural elements shown above the alignment (α helices, β strands, and loop regions). B–D, anti-Strep (A3B) co-IPs of the indicated Flag-tagged CDK constructs from 293T cells. See Fig. 2A legend for a description of the IP labeling scheme.
