Figure 6.
Effect of SQR on reactive cysteine modifications in the mitochondrial proteome. a, differential effects on cysteine reactivity in mitochondrial proteins modulated by SQR activity. L/H ratios for all identified mitochondrial cysteine-containing peptides corrected for changes in protein expression are shown; a subset is highlighted. Peptides with high levels of modified cysteine residues in the heavy sample (HT29SQRscr) display high L/H ratios. The data are the average of two independent experiments. b, pathway enrichment analysis of cysteine-containing peptides with L/H >2. Pathways exhibiting a >10-fold enrichment included several involved in energy metabolism.