Figure 9.

CRC cells are more resistant than a nonmalignant colon cell line to the antiproliferative effect of H₂S. a, effect of H₂S (0.3 mm) on the proliferation of HCECs and HT29 cells. Values for untreated HCECs and HT29 cells at t = 0 h were set at 1. The data (mean ± S.D. (error bars)) are representative of three independent experiments, each performed in triplicate. b, the proliferation rate of HCECs was compared with that of four other colon cancer cell lines as indicated in the absence (white bars) or presence of Na₂S (gray bars). Cells were treated with 0.1 mm Na₂S at time 0 and every 12 h, and the medium was changed every 24 h for a total treatment time of 72 h, as described under “Experimental procedures.” Proliferation was measured using the MTT assay. The data are representative of three independent experiments, each performed in triplicate, and represent the mean ± S.D.