Figure 2.

Gadolinium-sensitive, DVF-induced whole-cell currents are observed in pmp22^+/+ but not in pmp22^−/− mouse Schwann cells. A, average whole-cell currents recorded from WT mouse Schwann cells exposed to +divalents (left, +Ca²⁺/Mg²⁺), divalent-free (center, −Ca²⁺/Mg²⁺), or divalent-free plus 10 μm Gd³⁺ (right) solutions. B, average IVs for Gd³⁺-sensitive DVF-induced currents (●) and for currents measured in the presence of divalents (○) (n = 6). C, representative whole-cell currents recorded from pmp22^−/− mouse Schwann cells exposed to divalent-free (left) or divalent-free plus 10 μm Gd³⁺ (right) solutions. D, example of large currents recorded from some pmp22^−/− mouse Schwann cells exposed to divalent-free (left) or divalent-free plus 10 μm Gd³⁺ (right) solutions. E, average whole-cell currents measured at −60 mV and normalized for membrane capacitance in the presence of divalents, in DVF solution, and in DVF solution + 10 μm Gd³⁺. Left, no DVF-induced current (n = 8); right, Gd³⁺-resistant DVF-induced currents (n = 6). *, p < 0.05, **, p = 0.003; ***, p < 0.0001.