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. Author manuscript; available in PMC: 2019 Nov 1.
Published in final edited form as: Nature. 2019 May 1;569(7758):723–728. doi: 10.1038/s41586-019-1173-8

Extended Data Fig. 9: Inhibition of H3K27 trimethylation restores features of the CAF phenotype.

Extended Data Fig. 9:

(a) Expression of CAF marker genes in CAFs expressing shNNMT and treated with the EZH2 histone methyltransferase inhibitor DZNep. Two-sided t-test, n = 3 biological replicates. (b) Immunoblot of CAFs expressing a shNNMT construct treated with the general methyltransferase inhibitor 3-DZA. Knockdown of EZH2 in CAFs expressing a shNNMT construct leads to (c) decreased H3K27 trimethylation, and (d) increased collagen contractility. Two-sided t-test, n = 3 biological replicates. All bar graphs represent mean of data and error bars are SEM.