REAGENT or RESOURCE	SOURCE	IDENTIFIER
Antibodies and Dyes
pAkt (Ser473), h	Cell Signaling Tech.	CST #4058
pS6 (Ser235/236), h	Cell Signaling Tech.	CST #2211
Annexin V-APC	eBioscience	BMS306APC-20
Cyclin B1 (V152), h	Cell Signaling Tech.	CST #4135
XIAP, h	BD Biosciences	610762
Survivin, h	Cell Signaling Tech.	CST # 2808
MPM-2, h	Millipore	05–368
pBcl-2 (Ser70), h	Cell Signaling Tech.	CST #2827
Mcl-1, h	R&D Systems	MAB828
cl. Caspase 3 (Asp175), h	Cell Signaling Tech.	CST #9661
JC-1	Enzo Life Sciences	ENZ-52304
FITC anti-mouse CD5 Antibody	BioLegend	100606
PE anti-mouse CD2 Antibody	BioLegend	100107
PE Donkey Anti-Rabbit IgG	Jackson Immuno	711–116-152
APC Goat Anti-Mouse IgG	Jackson Immuno	115–135-164
BV421 Goat Anti-Rabbit IgG	BD Biosciences	565014
DAPI	Molecular Probes	D1306
Propidium Iodide	Molecular Probes	P3566
Fixable Viability Dye eFluor 780	eBioscience	65–0865-14
Alexa Fluor 488 NHS Ester	Molecular Probes	A20000
Alexa Fluor 546 NHS Ester	Molecular Probes	A20002
Acridine Orange	Sigma-Aldrich	235474
Biological Samples
Primary murine acute myeloid leukemia sample (JW81),female	Lab of Kevin Shannon	N/A
Chemicals, Peptides, and Recombinant Proteins
GDC-0941 (Pictilisib)	Selleckchem	S1065
IPI-145 (Duvelisib)	Selleckchem	S7028
DXR	Selleckchem	S1208
BKM120 (Buparlisib)	Selleckchem	S2247
XL147 (Pilaralisib)	Selleckchem	S7645
AS-605240	Selleckchem	S1410
EHop-016	Selleckchem	S7319
CTA 056	Tocris	4726/10
VCR	Selleckchem	S1241
Centrinone B	Tocris	5690/10
BX-795	Selleckchem	S1274
Dorsomorphin	Selleckchem	S7840
Defactinib	Selleckchem	S7654
ARRY-520	Tocris	4676/10
Mps1-IN-5	MedChem Express	HY-12858
GDC-0941	Genentech	N/A
Critical Commercial Assays
QIAamp DNA Blood Maxi Kit	QIAGEN	51192
PstI	New England Biolabs	R3140T
Silica column	Denville Scientific	CM-0600–20
HiFi Phusion polymerase	New England Biolabs	M0530L
Midi GeBAflex tubes	Gene Bio-Application	T010
Experimental Models: Cell Lines
JURKAT cells (human, male)	Lab of Jeroen Roose	N/A
MOLT3 cells (human, male)	Lab of Jeroen Roose	N/A
CCRFCEM cells (human, female)	Lab of Jeroen Roose	N/A
CUTLL1 cells (human, male)	Lab of Jeroen Roose	N/A
HPBALL cells (human, male)	Lab of Jeroen Roose	N/A
HUT78 cells (human, male)	Lab of Jeroen Roose	N/A
KOPTK1 cells (human, male)	Lab of Jeroen Roose	N/A
MOLT4 cells (human, male)	Lab of Jeroen Roose	N/A
MOLT13M cells (human, female)	Lab of Jeroen Roose	N/A
PEER cells (human, female)	Lab of Jeroen Roose	N/A
LN229 cells (human, female)	Lab of William Weiss	N/A
SF767 cells (human, female)	Lab of William Weiss	N/A
U87 cells (human, female)	Lab of William Weiss	N/A
U373 cells (human, male)	Lab of William Weiss	N/A
BT549 cells (human, female)	Lab of Zena Werb	N/A
HBL100 cells (human, female)	Lab of Zena Werb	N/A
HCC3153 cells (human, female)	Lab of Zena Werb	N/A
HS578T cells (human, female)	Lab of Zena Werb	N/A
MDAMB231 cells (human, female)	Lab of Zena Werb	N/A
T47D cells (human, female)	Lab of Zena Werb	N/A
BxPc3 cells (human, female)	Lab of Rushika Perera	N/A
KP4 cells (human, male)	Lab of Rushika Perera	N/A
Panc1 cells (human, male)	Lab of Rushika Perera	N/A
SW1990 cells (human, male)	Lab of Rushika Perera	N/A
HCT15 cells (human, male)	Lab of Jeroen Roose	N/A
HCT116 cells (human, male)	Lab of Jeroen Roose	N/A
SW48 cells (human, female)	Lab of Jeroen Roose	N/A
SW620 cells (human, male)	Lab of Jeroen Roose	N/A
Experimental Models: Organisms/Strains
F1 C57BL/6 × 129Sv/Jae mice	UCSF Laboratory Animal Resource Center, Breeding Core	N/A
C57BL/6 mice	Lab of Jeroen Roose	N/A
Oligonucleotides
TruSeq Index Primers (The barcoding primers are proprietary to Illumina and were shared with the UCSF Genomics Core with the constraint not to publish the individual sequences.)	Illumina	N/A
Recombinant DNA
UCSF EXPANDed RNAi library	Lab of Michael McManus, UCSF ViraCore	Bassik et al., 2009
Software and Algorithms
Galaxy		usegalaxy.org
Bowtie	Langmead et al., 2009	bowtie-bio.sourceforge.net
DESeq2	Love et al., 2014	(https://bioconductor.org/packages/release/bioc/html/DESeq2.html)
MAGecK	Li et al., 2014	(http://sourceforge.net/projects/mageck)
Ingenuity Pathway Analysis	QIAGEN Bioinformatics	N/A
RStudio	RStudio	v1.0.136
R	The R Project	v3.3.2