Table 4.

CNS proteomic studies in MS

	Study (Brain)	Number of patients	Main finding	Method	Ref
1	Pathogenesis	Autopsied brain samples of two female MS patients (acute MS) Immune response study: Serum from 20 additional MS patients, and seven control patients	Mutated forms ofProteolipid Protein 1, mutant-specific immuneresponse	SDS-PAGEnLC-MS/MSPeptide microarrays, NGS	[27]
2	Remyelination promoting proteins	Postmortem MS tissue	EphrinB3 target for therapies to promote remyelination in demyelinating disease	SDS-PAGE nLC-MS/MSLCM, in vitro/ in vivo study	[81]
3	Pathogenesis and/or remyelination Markers	Discovery set- 2 Postmortem brain blocks SPMS Validation-12PMS SPMS/PPMS	Thymosin β-4 in partially remyelinated lesions	H&E, LFB, MALDI-IMS, LC-MS/MS, IHC	[82]
4	Neuroprotection	Five postmortem MS cortical tissueand four control brains	Hemoglobin β subunit (Hbb) interacting proteins (ATP synthase, histones, and a histone lysine demethylase)	LC-MS/MS, neuronal cultures, IHC	[85]