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. Author manuscript; available in PMC: 2020 Aug 1.
Published in final edited form as: J Physiol. 2019 Jul 11;597(15):3817–3832. doi: 10.1113/JP277769

Figure 1. Preparation of mitoplast from left ventricle cardiomyocytes.

Figure 1

A) Healthy rat cardiomyocytes isolated with a Langendorff perfusion apparatus. B) Electron microscopy image of mitoplasts (inner mitochondrial membrane devoid of outer membrane), red arrow. A low percentage of intact swollen mitochondria remained (top-left panel B). C) Mitoplasts labeled with Mitotracker Red visualized with differential interference contrast (DIC) Nomarski microscopy through a 60X objective. The arrow in C indicate a selected mitoplast for patch-clamp. D) DIC image of panel C show a borosilicate patch pipette next to a mitoplast. E) Schematic representation of the preparation of mitoplasts. In an excised patch (inside-out configuration), the bath solution represents the mitochondrial matrix.