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. Author manuscript; available in PMC: 2020 Jul 1.
Published in final edited form as: Mol Cell Neurosci. 2019 Jun 12;98:140–154. doi: 10.1016/j.mcn.2019.06.004

Figure 2.

Figure 2.

Acrolein-lysine adducts increase in whole brain preparation, striatum, and substantia nigra after mild bTBI. (A) Western Blot image of acrolein-lysine modified proteins (FDP-Lys) and β-actin for each group at control, 2-day and 7-day post-blast. All bands on the acrolein-lysine blot were used for analysis for each group. (B) Quantification demonstrated significant difference between control, 2-day post-injury, and 7-day post-injury [F(2,22)=5.20, p=0.014]. (C) Western Blot image of acrolein-lysine modified proteins (FDP-Lys) and β -actin for each group at control, 2-day and 7-day post-blast of the B5 (anterior) striatal region. All bands on the acrolein-lysine blot were used for analysis for each group. (D) Quantification demonstrated significant difference between control, 2-day post-injury, and 7-day post-injury [F(2,9)=6.58, p=0.017]. (E) Western Blot image of acrolein-lysine modified proteins (FDP-Lys) and β-actin for each group at control, 2-day and 7-day post-blast of the C5 (posterior) striatal region. (F) Quantification demonstrated significant difference between control, 2-day post-injury, and 7-day post-injury [F(2,10)=6.22, p=0.018]. . Acrolein-lysine adducts increase in substantia nigra region after mild bTBI. (G) Western Blot image of acrolein-lysine modified proteins (FDP-Lys) and β -actin for each group at control, 2-day and 7-day post-blast. All bands on the acrolein-lysine blot were used for analysis for each group. (H) Quantification demonstrated significant differences between control, 2-day post-injury, and 7-day post-injury [F(2,10)=5.61, p=0.023]. Data are relative, normalized as percent control and presented as mean ± SEM. Tukey’s post-hoc test. n=8-9 (whole brain) and n=4-5 (striatum and substantia nigra). *p<0.05, **p<0.01.