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. 2019 Aug 8;12:2473–2485. doi: 10.2147/JPR.S214671

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© 2019 Kuthati et al.

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IIK-7 treatment reduces PSNT-induced microglia activation in rat spinal cords. (A) On day 6 after drug treatment, spinal cord sections were fixed and single-labeled with fluorescein isothiocyanate-labeled anti-OX42 antibodies (green; microglia) and the nuclei stained with 4ʹ,6-diamidino-2-phenylindole (DAPI) (blue); then, images were captured and merged by fluorescence microscopy. The sections are from (A) Vehicle-injected PSNT rats, (B) IIK-7-injected rats. The nuclei (blue lane 1) and microglia (green lane 2) are merged in lane 3. (B) The microglial activation is quantified by counting the number of activated microglial cells and expressed relative to the value for the vehicle-injected group. Data are analyzed by Tukey test (**p<0.01). The pictures are representative of those seen in samples from 3 rats.

Abbreviations: IIK-7, N-Butanoyl 2-(9-methoxy-6H-iso-indolo[2,1-a]indol-11-yl)-ethan-amine; PSNT, Partial sciatic nerve transection; DAPI, 4′,6-diamidino-2-phenylindole.