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. 2019 Aug 12;9:11667. doi: 10.1038/s41598-019-47055-1

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Expression of Sirt1, FOXO3a and Caspase-3 in kidney of normoglycemic and STZ-induced diabetic mice. (a) Western blot analysis of renal Sirt1 protein expression (n = 4, each group). (b) Western blot analysis of renal pFOXO3a ser318/321 and total-FOXO3a protein expression (n = 4, each group) (c) Western blot analysis of renal Caspase 3 protein expression (n = 5, each group). PRR, (Pro)renin receptor; Veh + Scr shRNA, vehicle with scrambled PRR shRNA; Veh + PRR shRNA, vehicle with PRR shRNA; DM + Scr shRNA, diabetes with scrambled shRNA; DM + PRR shRNA, diabetes with PRR shRNA. Data presented as mean ± SEM, *p < 0.05 vs. Veh + Scr shRNA; ^#p < 0.05 vs. DM + Scr shRNA.