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. 2019 Aug 13;10(8):611. doi: 10.1038/s41419-019-1860-2

Fig. 5. MLN4924 inhibits TNF-induced production of survival proteins.

Fig. 5

a KMS-12BM and MM.1S cells were primed with MLN4924 (20 µM) for 2 h or remained untreated. Cells were then stimulated with 300 ng/ml Fc-TNF(32W/86T) for 1, 2, 4, or 6 h (left panels) or for 6 h with 10, 30, 100, or 300 ng/ml Fc-TNF(32W/86T) (right panels). Total cell lysates were analyzed by Western blotting for the presence of the indicated proteins. b Cells were primed with MLN4924 and were then stimulated with Fc-TNF(32W/86T). The TNFR1-associated signaling complex was analyzed by help of protein G immunoprecipitation and Western blotting. Data shown are representative of at least two independent experiments