Fig. 7. Forced TNFR1 cell surface expression in KMS-11 cells engenders sensitivity for TNF/MLN4924-induced cell death.

a KMS-11 and KMS-11-TNFR1 cells were analyzed for TNFR1 cell surface expression by FACS. b Cells were challenged overnight as indicated in technical triplicates with TNF and MLN4924 (20 µM) and analyzed for cell viability. c KMS-11-TNFR1 cells were primed with MLN4924 (20 µM) for 2 h or remained untreated. Cells were then stimulated with the indicated concentrations of Fc-TNF(32W/86T) for 6 h and total cell lysates were analyzed by Western blotting for the presence of the indicated proteins. d The TNFR1-associated signaling complex of KMS-11-TNFR1 cells was analyzed after 10 min by protein G immunoprecipitation and Western blotting. Data shown are representative of at least two independent experiments