Fig. 5. Mitochondrial damage and increased ROX in MLP-deficient CMs.

a qPCR analysis of mitochondrial DNA (ND1 and ND2) to nuclear DNA (β-actin) ratio at days 15, 22, and 30 of cardiac differentiation (n = 9). b Mitotracker images and c, d quantification of Mitotracker green intensity obtained by flow cytometry demonstrates a significantly reduced fluorescence intensity in MLP KO hESC-CMs at day 30 as compared with WT hESC-CMs (n = 3). Scale bar, 50 μm. e–g Cell ROS green intensity and h–j MitoSox Red intensity suggests increase in ROS for MLP KO hESC-CMs as compared with WT hESC-CMs (n = 3). Scale bar, 50 μm. Results are presented as means ± S.E.M. of three independent experiments. **P < 0.01; ***P < 0.001; ****P < 0.0001; ns, not significant, unpaired two-sided Student’s t test