Fig. 6.

HFR1 physically interacts with HAC1 and regulates flowering by activating AG. a A sketch map of yeast strains on the selective plates. BD + AD, yeast strain AH109 transformed with empty vectors pGBK-T7 and pGAD-T7; HAC1-BD + AD, AH109 transformed with pGBK-T7 background vector expressing HAC1-BD and empty vector pGAD-T7; BD + HFR1-AD, AH109 transformed with pGBK-T7 empty vector and pGAD-T7 background vector expressing HFR1-AD; HAC1-BD + HFR1-AD, AH109 expressing HAC1-BD and HFR1-AD in pGBK-T7 and pGAD-T7 vectors respectively. b–d Growth of the strains streaked on selective media plates with SD/-Trp/-Leu (b), SD/-Trp/-Leu/-His/-Ade (c) and SD/-Trp/-Leu/-His/-Ade/ + 3-AT (d). e A mechanistic model for HFR1 and HAC1’s physical interaction to activate AG by histone acetylation