Table 1.
Results of the breast cancer cell lines.
| Cell line | HER2 IHC Agarcyto* | HER2 FISH Metaphase spreads (ratio)** | HER2 FISH Agarcyto (ratio)** | HER2 MLPA (ratio)*** | CEP17 FISH number of signalsMetaphase spreads | CEP17 FISH number of signals Agarcyto | Copy number chromosome 17 with MLPA | DNA ploidy status |
|---|---|---|---|---|---|---|---|---|
| MDA-MB231 | 0 | 1.19 | 0.88 | 1.23 | 4 | 4 | 2 | Polyploid |
| MDA-MB436 | 0 | 1.00 | 1.00 | 0.71 | 1 | 1 | 1 | Hypoploid |
| MCF7 | 1+ | 0.69 | 0.85 | 0.63**** | 3 | 3 | 2 | Hyperploid |
| HCC1937 | 2+ | 1.06 | 1.01 | 1.21 | 6 | 6 | 2 | Hyperploid |
| OCUB-F | 3+ | ≫2 | ≫2 | 7.78 | 4 | 4 | 2 | Hyperploid |
| SK-BR-3 | 3+ | ≫2 | ≫2 | 6.11 | 6 | 5 | 2 | Hyperploid |
*Scoring of 0, 1+, 2+ or 3+, **Ratio of spots for the HER2 gene and CEP17; mean value of 2 independent estimations; ***Ratio as mean value of both MLPA kits used; ****Partial loss of the HER2 gene.