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. 2019 Aug 12;9:11592. doi: 10.1038/s41598-019-48078-4

Figure 2.

Figure 2

Correction of Hematological Indices and Extramedullary Hematopoiesis. (A) Spleen weight in IUGT 12 Weeks animals was significantly lower 0.12 ± 0.0065 than untreated thalassemia controls 0.31 ± 0.017, n = 8, p < 0.0001 and similar to Humanised Non-Thalassemia Control 0.098 ± 0.0031, One Way ANOVA, Bonferroni’s Multiple comparisons test. (B) Red Blood Cell Count in IUGT 12 Week animals was significantly higher 9.36 ± 0.27 10^12/L, n = 8 than untreated thalassemia controls 6.23 ± 0.45 10^12/L, n = 8, p = 0.0015 and similar to Humanised non-thalassemia controls 8.47 ± 0.57 10^12/L, n = 8, p = 0.089, One Way ANOVA, Bonferroni’s Multiple comparisons test. (C) The Hematocrit in IUGT 12 Week animals was significantly higher 41.79 ± 1.76%, n = 8, than untreated thalassemia controls 27.19 ± 2.02, n = 8, p = 0.0025 and similar to Humanised non-thalassemia controls 41.76 ± 2.80, n = 8, p > 0.99, One Way ANOVA, Bonferroni’s Multiple comparisons test. (D) Extramedullary Hematopoiesis in the Spleen was significantly lower in IUGT 12 Week animals 6.57 ± 1.78, n = 4, versus uninjected thalassemia controls 23.10 ± 4.57, n = 3, p = 0.00075 and similar to Humanised non-thalassemia controls 3.69 ± 0.41, n = 6, p = 0.64. Similarly, in the Liver, the level of EMH was lower in the IUGT 12 Week animals 0.74 ± 0.042, versus uninjected thalassemia controls 5.99 ± 0.87, n = 5, p = 0.10 and comparable to Humanised non-thalassemia controls 0.75 ± 0.091, n = 4, p > 0.99, One Way ANOVA, Bonferroni’s Multiple comparisons test. (E) Hematoxylin & Eosin light microscope images showing areas of extramedullary hematopoiesis (arrows) in the Spleen (Scale Bar 100 μm) and Liver (Scale Bar 50 μm).