Figure 6.

Endothelial RhoA deficiency does not affect postnatal retinal angiogenesis. (A) Schematic diagram of inducible endothelial-RhoA deficient mice with the Cdh5 tamoxifen-inducible promoter Cdh5-BAC-Cre^ER+. (B) Experimental outline for retinal angiogenesis assay on postnatal day 6 (P6). (C,D) Representative images (C) and radial growth, number of filopodia quantifications and number of caved lesions per retina (D) of P6 CD31-stained retinal vessels between endothelial RhoA-deficient mice and littermate controls (n = 4). Empty arrowheads represent caved edges of the vascular fronts, observed in RhoA-deficient mice. (E) Experimental outline for retinal angiogenesis assay on P9. (F,G) Representative images (F) and quantification (G) of retinal vessels, distinguishing superficial and deep plexus stained with biotinylated isolectin B4 (IB4) (n = 3). **P < 0.01