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. 2019 Aug 12;9:11587. doi: 10.1038/s41598-019-47734-z

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Enhanced expression of P2X7 Receptor in TAMR-MCF-7 cells. (A) mRNA Expression of P2Y receptors and P2X receptors in MCF-7 and TAMR-MCF-7 cells. mRNA levels were determined by RT-PCR analyses. (B) Quantitative mRNA expression of P2X7 receptors in MCF-7, TAMR-MCF-7, and T47D cells (n = 11). (C) Protein Expression of P2X7 in MCF-7, TAMR-MCF-7, and T47D cells (n = 3). (D) ATP-induced P2X7 activation in MCF-7 and TAMR-MCF-7 cells. [Ca²⁺]i were measured after treating both the cell types with ATP (1–1000 μM) in ATP-free buffer condition (Left). MCF-7 and TAMR-MCF-7 cells were pretreated with 3 μM KN62, a selective P2X7 antagonist, and then incubated with ATP (10, 100 and 1000 μM) (Right)(n = 4). (E) Protein expression of inflammasome units (ASC, NLRP3 and pro-caspase-1) in MCF-7 and TAMR-MCF-7 cells (n = 4). (F) IL-1β and IL-18 production in culture media by detected ELISA analyses (N.S., not significant. N.D., not detected). All data represent the mean ± SE (*p < 0.05, ***p < 0.005, significant as compared to MCF-7 cells; ^# < 0.05, ^### < 0.005, significant as compared to TAMR-MCF-7 control group).