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. 2019 Aug 12;9:11587. doi: 10.1038/s41598-019-47734-z

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Limited role of P2X7 in cell proliferation of MCF-7 and TAMR-MCF-7 cells. (A) Effects of ATP on cell proliferation of MCF-7 and TAMR-MCF-7 cells (n = 5). (B) Effects of KN62 (0.3–3 μM) on cell proliferation of MCF-7 and TAMR-MCF-7 cells (n = 6). (C) Effects of KN62 (0.3–3 μM) cotreatment on cell proliferation of MCF-7 and TAMR-MCF-7 cells incubated with 100 μM ATP (n = 6). (D) Inhibition of P2X7 expression by P2X7 siRNA treatment. (E) Effects of P2X7 silencing on cell proliferation of MCF-7 and TAMR-MCF-7 cells (n = 10). All data represent the mean ± SE (^###p < 0.005, significant as compared to TAMR-MCF-7 control group).